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Bifidobacterium infantis 35624 (Align)

Strain-specific probiotic with the cleanest RCT signal for global IBS symptom improvement.

Why

Bifidobacterium infantis 35624 (sold as Align in the US and as Symprove and others in Europe) is the bacterial probiotic with the most consistent RCT signal for irritable bowel syndrome. The Whorwell 2006 RCT demonstrated significant improvements in pain, bloating, bowel dysfunction and global IBS symptoms over 4 weeks. Subsequent trials and a strain-specific meta-analysis confirmed the signal at the 10⁸ CFU/day dose.

How it works

Modulates intestinal cytokine balance, restores the IL-10:IL-12 ratio that is reduced in IBS. Surface exopolysaccharides inhibit pathogen adhesion. Bile-acid metabolism modulation may underlie part of the bowel-function effect.

Expected onset · Symptom improvement typically over 4 weeks of continuous use

How to take

Dosage

1 × 10⁸ CFU/day (one Align capsule), continuous use for at least 4 weeks before assessing benefit.

Timing

Once daily, with or without food

On the label

Specifically B. infantis 35624 (also marketed as B. longum subsp. infantis 35624). Generic 'Bifidobacterium' products are not equivalent.

Ideal for

Adults with IBS (any subtype, including mixed) seeking a strain-specific probiotic with direct RCT evidence.

Safety

Generally very well tolerated. Avoid in critically ill or immunocompromised patients (general probiotic precaution). No significant drug interactions documented.

Evidence

At a glance

Whorwell 2006 Am J Gastroenterol RCT (n=362 IBS women): B. infantis 35624 at 10⁸ CFU/day significantly improved global IBS symptom score, pain, bloating and bowel-function over 4 weeks. Yuan 2017 strain-specific meta-analysis confirmed signal. Effect modest but distinct in being one of the few probiotic indications with direct strain-level RCT evidence.

Where to get it

Shop Bifidobacterium infantis 35624 (Align) on Amazon

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